Journal: The Journal of pharmacology and experimental therapeutics

Article Title: Empagliflozin Protects against Pulmonary Ischemia/Reperfusion Injury via an Extracellular Signal-Regulated Kinases 1 and 2-Dependent Mechanism.

doi: 10.1124/jpet.121.000956

Figure Lengend Snippet: Fig. 5. The effect of empagliflozin on protein phosphorylation after I/R injury. Left: Western blots of p-GSK-3b and t-GSK-3b (A), p-AKT and t-AKT (B), p-ERK1/2 and t-ERK1/2 (C), p-STAT-1 and t-STAT-1 (D), p-STAT-3 and t-STAT-3 (E), p-STAT-5 and t-STAT-5 (F) in lung tissues after I/R injury. Right: bar graph showing mean ratio of the phospho-/total band densities. **P < 0.01, ***P < 0.001, versus sham-operated mice; ###P < 0.001, versus control mice. n 5 5 per group.

Article Snippet: The primary antibodies used in this study were: p-glycogen synthase kinase-3b (GSK-3b), phosphorylated GSK-3b (Ser9); t-GSK-3b, total GSK-3b; p-protein kinase B (AKT), phosphorylated AKT (ser473); t-AKT, total AKT; pERK, phosphorylated ERK 1/2 (Thr202/Tyr204); t-ERK, total ERK1/2; p-signal transducer and activator of transcription (STAT)-1, phosphorylated STAT-1 (Tyr701); t-STAT-1, total STAT-1; P-STAT-3, phosphorylated STAT-3 (Tyr705); t-STAT-3, total STAT-3; p-STAT-5, phosphorylated STAT-5 (Tyr694); t-STAT-5, total STAT-5 (all primary antibodies, 1:1000, Cell Signaling, Danvers, MA).

Techniques: Phospho-proteomics, Western Blot, Control